Equilibrium folding intermediates of a greek key β-barrel protein
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چکیده
منابع مشابه
Equilibrium folding intermediates of a Greek key beta-barrel protein.
Protein S is a calcium-binding protein comprising two Greek key beta-barrel domains. We have used NMR and optical spectroscopies to show that, in the absence of calcium, the N-terminal domain of protein S forms two equilibrium folding intermediates that are in slow exchange. The intermediates arise from differential calcium-dependent folding of subdomains which are not contiguous along the poly...
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To determine the pathway by which a protein folds up, it is necessary to characterize the structures of folding intermediates and also to place these intermediates in the correct order on the kinetic pathway of folding. Noncovalent folding reactions are fast: typically they occur in seconds or less for small single-domain proteins. On the other hand, hours are required to obtain detailed struct...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 1998
ISSN: 0022-2836
DOI: 10.1006/jmbi.1997.1563